LOCUS       pLV-[hsa-miR-544a]       9169 bp    DNA     circular     27-JUN-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7090..7950
                     /label=Amp(R)
     promoter        8151..8691
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5695..5929
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6504..6959)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    6001..6131
                     /label=SV40 pA
     misc_feature    6132..6345
                     /label=SV40 ori
     misc_feature    5016..5612
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3634
                     /label=intron
     CDS             3681..5006
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3424
                     /label=hsa-mir-544a precursor
BASE COUNT     2164 a      2367 c      2491 g      2147 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatcca agacgttgtg 
     3121 gtcgacagca cctctggggt gtgtgtgtgt gtgtgtgtgt gtgtgtttgt gtgcgtgtgc 
     3181 atatgaattg ttggctagag tagtgtttct caaactttaa aaatgctcct aagattttca 
     3241 tcacctaggg atcttgttaa aaagcagatt ctgattcagg gaccaagatt ctgcattttt 
     3301 agcaagttct caagtgatgc taataccgcc ggtctgtgga caacactcac agtggtttgg 
     3361 ggctggagac caggtgcttt ccctgtgata tgtgtcgcct gtctccccac atgccatata 
     3421 ccgtgctagc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc 
     3481 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat 
     3541 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca 
     3601 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaaaactac ccctctagag 
     3661 tcgagctacc ggtcgccacc atggtgagca agggcgagga ggataacatg gccatcatca 
     3721 aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga 
     3781 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga 
     3841 ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct 
     3901 ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg 
     3961 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc 
     4021 aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact 
     4081 tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc 
     4141 ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg 
     4201 acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc 
     4261 tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca 
     4321 ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc 
     4381 tgtacaagga cccaccggtc gccaccatga ccgagtacaa gcccacggtg cgcctcgcca 
     4441 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg 
     4501 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac 
     4561 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg 
     4621 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg 
     4681 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc 
     4741 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc 
     4801 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg 
     4861 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg 
     4921 agcggctcgg cttcaccgtc accgccgacg tcgagtgccc gaaggaccgc gcgacctggt 
     4981 gcatgacccg caagcccggt gcctgagcgg ccgcgtcgac aatcaacctc tggattacaa 
     5041 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata 
     5101 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc 
     5161 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg 
     5221 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac 
     5281 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat 
     5341 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt 
     5401 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat 
     5461 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc 
     5521 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag 
     5581 tcggatctcc ctttgggccg cctccccgcc tggaattcga gctcggtacc tttaagacca 
     5641 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa 
     5701 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg 
     5761 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 
     5821 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 
     5881 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca 
     5941 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg 
     6001 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 
     6061 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 
     6121 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc 
     6181 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 
     6241 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 
     6301 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt 
     6361 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc 
     6421 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt 
     6481 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 
     6541 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 
     6601 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 
     6661 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 
     6721 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 
     6781 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 
     6841 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 
     6901 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta 
     6961 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 
     7021 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 
     7081 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 
     7141 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 
     7201 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 
     7261 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 
     7321 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 
     7381 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 
     7441 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 
     7501 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 
     7561 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 
     7621 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 
     7681 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 
     7741 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 
     7801 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 
     7861 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 
     7921 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 
     7981 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 
     8041 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 
     8101 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 
     8161 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 
     8221 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag 
     8281 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 
     8341 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 
     8401 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 
     8461 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 
     8521 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 
     8581 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 
     8641 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 
     8701 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 
     8761 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt 
     8821 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag 
     8881 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 
     8941 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat 
     9001 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg 
     9061 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac 
     9121 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgc 
//
